DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time
نویسندگان
چکیده
Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation disappearance of double-strand breaks (DNA DSB) as a function LET time. Comparisons conventional ?-rays high carbon ions were also performed.Materials Methods In the present work, performed immunofluorescence-based assay determine amount DSB induced by different values along 62 MeV proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma HTB177 non-small lung cells. Time dependence foci was followed well. irradiation positions, corresponding desired values, numerical simulations carried using Geant4 toolkit. We compared ?-H2AX persistence protons that ions.Results With rise SOBP, increase number detected lines up distal end while there decrease on its fall-off part. prolonged incubation time, gradually drops tending attain residual level. For maximum DSB, higher level than ?-rays. Carbon produce more but substantially. produced significantly lower particularly ions. do considerably it could be expected due their physical properties.Conclusions situ visualization reveal creation lesions clinically relevant SOBP lack significant differences ion-irradiated samples suggests an increased complexity slower repair kinetics protons. all types, no major difference shortly irradiations, later on, starts express inherent nature tested cells, therefore increasing discrepancy them.
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ژورنال
عنوان ژورنال: International Journal of Radiation Biology
سال: 2021
ISSN: ['1362-3095', '0955-3002']
DOI: https://doi.org/10.1080/09553002.2021.1948140